Ocimum sanctum Linn. Ethanolic extract promotes an antiproliferative and apoptosis activity in MCF-7 and T47D breast cancer cell lines mediated by upregulation of ROS/RNS, Caspase 9, and Caspase 3: an in silico and in vitro study

Breast cancer is the most serious disease affecting women worldwide. Recently, breast cases reached 2.2 million people. The treatment method still developing. In addition, use of herbal medicine as a palliative therapeutic to chemical and/or synthetic drugs increasing. Ocimum sanctum Linn. popular plant in Indonesia and Southeast Asia countries also known an medicinal plant. study aimed prove ability ethanolic extract (EEOS) antiproliferative against cancer. Cytotoxic assay, adhesion Reactive Nitrogen Species (RNS) production determined MCF-7 T47D cell. Furthermore, SEM applied visualize cell morphology. molecular docking performed. result shows EEOS inhibited proliferation MCF7 cells line. Surface morphology showed that tend be apoptotic (cells turned rougher, gritty, blebbing). increased RNS production. Molecular describes phytochemical compounds on (gallic acid, caffeic rosmarinic acid apigenin) interacted with caspase-3 caspase-9. conclusion, can inhibit lines correlate upregulated production, well expression Caspase 3 9.